- Real time PCR services
- Multigene panels
- microRNA services
- RNA isolation and QC
- Reverse transcription
- Data analysis
- Project consultation
Real time PCR services
|TaqMan® assay:||$225 per gene assay (inventoried)|
|$325 per gene assay (made to order)|
|SYBR® Green assay:||$40 per gene assay|
|Real time PCR:||$15 per sample per gene, triplicate wells|
|$12.50 duplicate wells|
|TaqMan® microRNA assay:||$200 per miRNA assay|
Our experienced scientists will select the best type of gene expression assay to meet your experimental needs as well as your budget, from SYBR® Green-based assays designed & validated onsite to pre-validated TaqMan® assays available from Applied Biosystems. Real-time PCR is performed with all the proper controls using the 7900 HT real-time PCR system.
One time $1200 per gene panel* set up charge for up to 90 genes of interest and 5 client-specified endogenous control genes.
Includes assay design, RNA isolation, RNA integrity assessment, cDNA synthesis, real-time PCR and data analysis for the first 2 samples.
Additional samples $200 per sample per panel. Price includes sample preparation, real-time service and data analysis as specified above.
For those clients wishing to profile multiple genes at once in the most cost effective manner, we offer SYBR® Green based multi-gene panels, designed to YOUR specifications. Whether a published pathway or a panel of your own choosing, you won't be restricted to specific gene sets or charged extra for custom work.
Even better, once you have purchased your panel, subsequent runs can be performed at a fraction of the cost of other commercial offerings, making essential experimental validation through replicates an affordable reality.
*Need less than a full panel of genes? Contact us to discuss the scope of your project and discounts available for smaller gene sets.
|TaqMan® microRNA assays:||$200 per miRNA assay|
|microRNA cDNA synthesis:||$10 per sample|
We use validated TaqMan® miRNA assays to bring unparalleled accuracy and sensitivity to your microRNA studies, from single miRNAs to wide profiles.
Our knowledgeable staff will work directly with you to determine the proper combination of endogenous control genes to insure the most accurate, reliable and reproducible results.
RNA isolation and QC
|RNA Isolation:||$30 per sample|
|RNA Integrity Assessment:||$30 per sample|
Our staff can isolate total RNA from cells or tissues by methods appropriate to your project. Extracted RNA can be returned to the client.
Initial assessment of RNA purity and concentration is performed by NanoDrop spectrophotometer analysis.
RNA integrity is determined by using the Agilent 2100 Bioanalyzer (optional but highly recommended).
Formalin-fixed, paraffin-embedded (FFPE), laser capture microdissection (LCM) and fine needle aspiration biopsy (FNAB) samples will require specialized isolation procedures and may incur additional cost. Please contact us for requirements and quotes on these specialized sample types.
|cDNA synthesis:||$10 per sample|
|microRNA synthesis:||$20 per sample|
Complementary DNA synthesis using proven, consistent and reliable reagents from established manufacturers.
In most cases, results are obtained and sent the client via e-mail within 1 week of receiving the sample.
The client's complete data analysis report will consist of:
- Raw Ct values
- ddCt analysis
Excel spreadsheet, including raw data, quantified data, ready-for-presentation graphs and statistical information (where applicable).
Materials and methods are available upon request.
$100 Initiation fee per project.
The initiation fee may be waived for larger orders and returning clients. Please contact us for more details.