ARQ Genetics provides rapid and reliable qPCR services (real-time PCR services, quantitative PCR services, and RT-qPCR services) for gene expression analysis, microRNA profiling, and gene copy number determination. Our custom qPCR solutions include RNA isolation, RNA quality assessment, cDNA synthesis, TaqMan and SYBR Green assay design and validation, qPCR optimization, and complete data analysis with normalization to your selected reference genes. All experiments are performed by Ph.D.-level scientists with years of molecular biology experience.
Why Choose ARQ Genetics as Your qPCR Service Provider?
As a specialized qPCR service provider, ARQ Genetics combines PhD-level expertise with state-of-the-art instrumentation to deliver accurate, publication-quality gene expression data. Unlike generalist core facilities or contract research organizations, we focus exclusively on real-time PCR services, allowing us to provide unmatched expertise and personalized attention to every project.
When selecting a qPCR service provider, researchers need more than just equipment access—they need scientific partners who understand the nuances of gene expression analysis. Our team brings decades of combined molecular biology experience to every experiment, from initial consultation through data delivery. We don’t just run samples; we actively collaborate to ensure your experimental design is optimized for success.
What Sets Us Apart as Your qPCR Service Provider:
PhD-Level Scientific Expertise: ALL of our research staff hold doctoral degrees in molecular biology or related fields, ensuring every project benefits from deep scientific understanding, not just technical execution.
True Flexibility: We design custom gene panels to YOUR specifications—choose exactly the genes you want to study, not pre-selected gene sets. No minimum orders, no restrictions, no compromises.
Comprehensive Service: From RNA isolation through complete data analysis, we handle every step with consistent quality and expertise. Our integrated workflow eliminates the coordination headaches of working with multiple vendors.
Fast Turnaround: Most results delivered within 5-7 business days, with expedited options available when your timeline is critical. We understand that research progress depends on timely data.
Transparent Communication: It’s not a “black box” service. We keep you informed at every step and welcome your input throughout the process. Our flexibility adapts to your evolving research needs.
Whether you need validation of a single gene across a few samples or comprehensive profiling of complex gene panels, ARQ Genetics provides the expertise, flexibility, and quality that advances your research. We’re proud to serve as the trusted qPCR service provider for researchers at universities, hospitals, and biotech companies nationwide.
Our qPCR Service Process
From sample receipt to data delivery, we handle every step with rigorous quality control and expert attention:
Step 1: Sample Receipt & RNA Extraction (1-2 days)
Total RNA isolation using methods appropriate to your sample type (cells, tissues, FFPE, LCM, FNAB), with NanoDrop quantitation and optional Agilent Bioanalyzer integrity assessment.
Step 2: Assay Design & Validation (3-5 days for custom SYBR Green)
Custom SYBR Green primer design and validation, or TaqMan assay selection. All primers validated for specificity with dissociation curve analysis to verify single product amplification.
Step 3: cDNA Synthesis & qPCR Analysis (3-4 days)
Reverse transcription with genomic DNA removal, followed by real-time PCR performed in triplicate on our 7900 HT and CFX-384 systems. All experiments include no-RT controls and proper reference genes.
Step 4: Data Analysis & Delivery (1 day)
ΔΔCt analysis normalized to your selected reference genes, with comprehensive report including raw Ct values, fold-change calculations, publication-ready graphs, and statistical analysis delivered via email.
Standard turnaround: 5-7 business days from sample receipt to data delivery.
Expedited service available for urgent projects.
Transparent qPCR Service Costs
Understanding qPCR service costs is essential for planning your research budget effectively. Unlike some service providers that obscure pricing or add hidden fees, we believe in complete transparency. Our straightforward pricing structure reflects the true value of expert gene expression analysis—combining PhD-level scientific expertise, state-of-the-art instrumentation, rigorous quality control, and comprehensive data analysis.
What Affects qPCR Service Cost?
Several factors influence the cost of professional qPCR services:
Detection Method: TaqMan probe-based assays provide superior specificity and are ideal for challenging targets, while SYBR Green dye-based assays offer cost-effective solutions for validated targets and large-scale screening.
Sample Processing: If we’re starting from cells or tissue, RNA isolation and quality assessment add to the total project cost but ensure optimal starting material for accurate results.
Number of Genes: Individual gene assays versus multi-gene panels each have different pricing structures. Our custom panel approach provides significant cost savings for researchers studying multiple genes simultaneously.
Sample Quantity: Larger sample sets benefit from our per-sample pricing model, making comprehensive studies more affordable than processing small batches repeatedly.
The True Cost of qPCR: Beyond the Price Tag
When evaluating qPCR service costs, consider the complete value equation. Running qPCR in-house requires significant capital investment in equipment ($30,000-$100,000+), ongoing maintenance costs, reagent inventory management, personnel training time, and the opportunity cost of your research team’s focus. Institutional core facilities may advertise lower per-sample costs due to subsidies, but often involve longer turnaround times, limited flexibility, and less specialized expertise.
ARQ Genetics provides transparent, competitive qPCR service costs that include everything you need for publication-quality results: expert consultation, optimized assay design, comprehensive quality control, complete data analysis, and ongoing support. No hidden fees, no minimum orders, no surprises—just straightforward pricing that reflects the true value of professional gene expression analysis.
Our Pricing Philosophy
We structure our qPCR service costs to be accessible for academic researchers while maintaining the quality standards demanded by industry clients. Whether you’re a graduate student validating dissertation results or a biotech company developing the next breakthrough therapy, you’ll receive the same expert attention and rigorous quality that has made us a trusted partner for gene expression analysis.
Review our detailed pricing below, and don’t hesitate to contact us for a customized quote tailored to your specific research needs. We’re happy to discuss how to optimize your experimental design for both scientific rigor and cost-effectiveness.
Real time PCR (qPCR) services
| TaqMan® assay: | $225 per gene assay (inventoried, up to 100 samples) |
| $325 per gene assay (made to order, up to 100 samples) | |
| SYBR® Green assay: | $50 per gene assay (one time cost for up to 1000 samples) |
| Real time PCR (qPCR): | $16.50 per sample per gene, triplicate wells |
| $15 duplicate wells | |
| TaqMan® microRNA assay: | $300 per miRNA assay (up to 100 samples) |
Our experienced scientists will select the best type of gene expression assay to meet your research needs as well as your budget, from SYBR® Green-based assays designed & validated onsite to pre-validated TaqMan® assays available from Applied Biosystems. qPCR is performed with all the proper controls using the CFX-384 real-time PCR (qPCR) system. We keep it simple: you tell us which genes you would like to analyze, send us your samples and we will do the rest! But, it’s not a “black box” service. We will keep you informed during all steps of the process and welcome your input. We know things change along the way and we are proud of our flexibility in meeting your evolving needs.
Multigene panels
One time $1200 per gene panel* set up charge for up to 90 genes of interest and 5 client-specified endogenous control genes.
Includes assay design, RNA isolation, RNA integrity assessment, cDNA synthesis, real-time PCR (qPCR) and data analysis for the first 2 samples.
Additional samples $200 per sample per panel. Price includes sample preparation, real-time service and data analysis as specified above.
For those clients wishing to profile multiple genes at once in the most cost effective manner, we offer SYBR® Green based multi-gene panels, designed to YOUR specifications. Whether a published pathway or a panel of your own choosing, you won’t be restricted to specific gene sets or charged extra for custom work.
Even better, once you have purchased your panel, subsequent runs can be performed at a fraction of the cost of other commercial offerings, making essential experimental validation through replicates an affordable reality.
*Need less than a full panel of genes? Contact us to discuss the scope of your project and discounts available for smaller gene sets.
microRNA services
| TaqMan® microRNA assays: | $300 per miRNA assay |
| microRNA cDNA synthesis: | $20 per sample |
We use validated TaqMan® miRNA assays to bring unparalleled accuracy and sensitivity to your microRNA studies, from single miRNAs to wide profiles.
Our knowledgeable staff will work directly with you to determine the proper combination of endogenous control genes to insure the most accurate, reliable and reproducible results.
RNA Isolation and QC
| RNA Isolation: | $40 per sample |
| RNA Integrity Assessment: | $30 per sample |
Our staff can isolate total RNA from cells or tissues by methods appropriate to your project. Extracted RNA can be returned to the client.
Initial assessment of RNA purity and concentration is performed by NanoDrop spectrophotometer analysis.
RNA integrity is determined by using the Agilent 2100 Bioanalyzer (optional but highly recommended).
Formalin-fixed, paraffin-embedded (FFPE), laser capture microdissection (LCM) and fine needle aspiration biopsy (FNAB) samples will require specialized isolation procedures and may incur additional cost. Please contact us for requirements and quotes on these specialized sample types.
Data Delivery & Reporting
Your comprehensive qPCR service report includes:
– Complete data analysis with fold-change calculations and statistical significance
– Raw Ct values for all samples, genes, and reference controls
– Primer sequences and validation data (for SYBR Green assays)
– RNA quality metrics (concentration, purity, integrity scores)
– qPCR amplification efficiency and dissociation curves (SYBR assays only)
– Publication-ready graphs and tables formatted for your needs
– All data delivered via email in Excel and PDF formats
– Technical support available for data interpretation and questions
– Any other questions? Please see our FAQ here
Sample Submission
RNA
If you are submitting previously isolated RNA, please provide at least 1µg of total RNA for individual genes or miRNAs and the same amount per panel/sample for gene panels. Please provide an assessment of RNA quality or take advantage of our RNA quality check service.
Note that for samples to be used for miRNA studies, a method that preserves small RNA, such as TriZol®, must be used. We do not recommend column-based isolation or methods that enrich the small RNA population as these may either result in loss of this fraction or adversely affect relative quantities between samples.
cDNA
If you have previously synthesized complementary DNA, we can utilize this material for gene expression experiments. Please insure that at least 20ng total RNA/µL was used during synthesis.
However, we recommend this approach only when a source of new tissue/cells or RNA is not available because varying protocols and reagents outside our control may produce inconsistent results.
Laser capture microdissection (LCM) samples
Please capture cells on commercial capture products (such as Arcturus Capsure® caps) and ship samples frozen on dry ice.
Formalin-fixed, paraffin-embedded (FFPE) samples
If available, please submit at least four, 20-micron sections, shipped at ambient temperature, specifying sample age, method of fixation and special requirements, if any.
Fine needle aspiration biopsy (FNAB) samples
Please store and ship samples on dry ice.
