qPCR services as a cost saving adjunct to NGS experiments

How does qPCR help in preventing unnecessary experiment repetitions?

qPCR helps prevent unnecessary experiment repetitions in several ways:

Precise Quantification: qPCR accurately determines library concentrations for NGS experiments, enabling optimal normalization between multiple samples. This precision ensures the correct amount of template is loaded onto the NGS platform, preventing low efficiencies or complete run failures that would necessitate repeats^[1].
Quality Control: By serving as a pre-NGS quality check, qPCR verifies cDNA integrity before sequencing, reducing the risk of failed runs that would require repetition^[2].
Early Contamination Detection: Including proper controls in qPCR experiments helps identify contamination before it’s too late, saving time and money by avoiding the need to repeat entire experiments^[2].
Rapid Validation: qPCR provides a fast and cost-effective means to confirm NGS findings, especially for specific genes of interest. This quick verification can prevent the need for more extensive and costly repetitions of NGS experiments^[3].
Technical Replicates: By using technical replicates in qPCR, researchers can protect their data. If one amplification fails, other wells may succeed, reducing the likelihood of having to repeat the entire experiment^[4].
Outlier Detection: Technical replicates in qPCR allow for potential outlier detection and removal, improving data quality and reducing the need for experiment repetition due to anomalous results^[4].

By leveraging these features of qPCR, researchers can significantly enhance the reliability and efficiency of their experiments, minimizing the need for costly and time-consuming repetitions.

Here at ARQ Genetics, our staff is ready to assist you with all steps in your vital NGS validation, so get in touch with one of our talented scientists today!