Frequently Asked Questions

Here is a graphic that describes the steps we take with your sample.

ALL of our research staff hold a Ph.D. in molecular biology or related field. Our clients can be assured that our scientists apply their years of gene expression experience to consistently deliver high quality data.

For experiments using in stock reagents, we strive to return your data within 5 business days. Back-ordered or custom items may take slightly longer. If you need your results sooner, we can expedite your order for a modest fee. Please contact us for details.

Your results will be delivered to an e-mail address you specify when you submit your sample. Data will be returned in the form of Excel spreadsheet(s) with all the standard error and basic statistical calculations performed, if applicable and requested.

An example of the data format from real time PCR experiments with individual genes can be viewed here.

An example of gene panel / pathway experiments spreadsheets can be viewed here.

Core facilities are typically supported by the host institution or grant funding and so may often charge a lower price to the client than comparable commercial services due to lower overhead. However, base cost is only one factor that needs to be considered when undertaking a project. Private companies typically have more incentive to see that the client gets what they want, more experience, a faster turn-around time and better ongoing support. If quality and reliability are as important as absolute cost to you, then a commercial firm may be more cost-effective in the long run. For more information and details, please see our blog post on the subject here.

We can certainly perform your experiments with less however, be aware that genes express at a low level may not be detected at lower concentrations of starting RNA material. Please contact us so we can get the most out of your available sample.

We can only keep samples on hand for six months from the time of your initial order due to storage space limitations. Follow-up experiments may be performed on those samples if the request occurs within six months of sample submission. After six months, you will either need to resubmit new samples or request that your old samples be returned at the conclusion of your initial order if you think you will need them for future experiments at a later date. Shipping & handling charges apply for samples that are returned.

Good question! We have covered this common question in our blog already. Please see the post here. But here’s a quick recap below.

Choosing between TaqMan and SYBR Green assays depends on several factors:

Choose SYBR Green when:

• Budget is a primary concern, especially for large-scale studies
• You’re screening multiple genes across many samples
• Working with well-characterized targets
• Running high sample numbers where per-gene setup cost matters

Choose TaqMan when:

• Specificity and accuracy are paramount (clinical samples, regulatory studies)
• Analyzing genes with low expression levels (like RNAi experiments)
• Working with genes that have similar sequences or splice variants
• Multiplexing is required to conserve precious samples
• You need minimal optimization time
• You need to analyze a SNP (Single Nucleotide Polymorphism)

Still unsure? Our PhD scientists offer free project consultations to evaluate your specific needs, sample types, target genes, and budget to recommend the best approach. We can also provide cost comparisons for your particular project. Contact us to get expert guidance.

We accept a wide variety of sample types for qPCR gene expression analysis:

Standard samples:

• Cultured cells (adherent or suspension)
• Fresh or frozen tissue samples
• Previously isolated total RNA

Specialized samples:

• Formalin-fixed, paraffin-embedded (FFPE) tissue
• Laser capture microdissection (LCM) samples
• Fine needle aspiration biopsy (FNAB) samples
• Blood, serum, or plasma

Sample requirements:

• For individual genes: At least 1µg total RNA per gene panel/sample
• For previously isolated RNA: We recommend assessment of RNA quality using our RNA integrity service
• For microRNA studies: RNA must be isolated using methods that preserve small RNA (such as TRIzol®)

Specialized sample types like FFPE, LCM, and FNAB require customized isolation procedures. Please contact us with details about your sample type so we can provide specific requirements and a quote.

We also offer RNA isolation services ($40 per sample) with optional RNA integrity assessment using the Agilent 2100 Bioanalyzer to ensure optimal starting material for your experiments.

Yes! Custom assay design is one of our specialties.

For SYBR Green assays: We design and validate custom primers onsite for your specific genes of interest. Our scientists use industry-leading design software and optimization protocols to ensure high specificity and efficiency. SYBR Green assays cost just $50 per gene (one-time cost for up to 1,000 samples).

For TaqMan assays: We offer both:

• Pre-validated TaqMan assays from Applied Biosystems inventory ($225 per gene, up to 100 samples) – available for thousands of well-characterized genes
• Custom made-to-order TaqMan assays ($325 per gene, up to 100 samples) – designed for unique sequences, splice variants, or proprietary targets

Multi-gene panels: We specialize in custom SYBR Green-based multi-gene panels designed to YOUR specifications. Whether analyzing a published pathway or a panel of your own choosing, you won’t be restricted to pre-defined gene sets. One-time setup: $1,200 for up to 90 genes plus 5 endogenous controls (includes design, validation, and analysis of first 2 samples).

Our experienced scientists will work with you to design primers and assays that span exon junctions (to avoid genomic DNA amplification), target specific splice variants, or meet other project-specific requirements.

Contact us to discuss your custom assay needs.

Yes! We provide complimentary qPCR gene expression project consultations with our PhD scientists.

What we help with:

• Experimental design and assay selection (TaqMan vs. SYBR Green)
• Sample preparation guidance and requirements
• Endogenous control gene selection for your tissue/cell type
• Statistical analysis approach and proper controls
• Troubleshooting and optimization strategies
• Cost-effective solutions for your budget

Why consult with us? ALL of our research staff hold Ph.D. degrees in molecular biology or related fields. We bring years of gene expression experience to every project and can help you avoid common pitfalls, optimize your experimental design, and ensure publication-quality results.

It’s not a “black box” service – we keep you informed during all steps of the process and welcome your input. We’re proud of our flexibility in meeting your evolving research needs.

Whether you’re just starting to plan your gene expression study or need expert advice on a challenging experiment, we’re here to help. There’s no obligation and no charge for consultation.

Ready to discuss your project? Contact us or call 512-308-1511 to speak with one of our scientists.

qPCR service costs vary based on the type of assay, number of samples, and complexity of your project. At ARQ Genetics, we offer transparent pricing with no hidden fees:

Standard qPCR Service Costs:

• SYBR Green assays: $50 per gene (one-time cost for up to 1,000 samples) – our most cost-effective option for large sample sets
• TaqMan assays: $225 per gene (inventoried assays, up to 100 samples) or $325 per gene (custom made-to-order assays)
• Per-sample analysis: $16.50 per sample per gene (triplicate wells) or $15 (duplicate wells)

Multi-Gene Panel Costs:

For researchers profiling multiple genes, we offer custom SYBR Green-based panels at exceptional value: $1,200 one-time setup (up to 90 genes plus 5 endogenous controls), with additional samples at $200 each. This is significantly more cost-effective than commercial platforms for large gene sets.

What’s Included in Our qPCR Service Cost:

Unlike some providers, our pricing includes comprehensive services with no surprise charges: assay design and validation, all reagents and consumables, expert data analysis with ΔΔCt calculations, proper controls and quality checks, and publication-ready results delivered within 5 business days. Optional add-ons like RNA isolation ($40/sample) and RNA integrity assessment ($30/sample) are clearly priced.

Comparing qPCR Service Costs:

When evaluating qPCR service costs, consider the total value. In-house qPCR requires equipment investment ($30,000-$100,000+), maintenance contracts, reagent inventories, personnel training, and troubleshooting time. Many researchers find our transparent pricing more cost-effective, especially for occasional or moderate-volume projects.

We offer free project consultations to provide accurate cost estimates tailored to your specific research needs. Contact us to discuss your project and receive a detailed quote.

Several key factors influence qPCR service costs, and understanding these helps you budget accurately and choose the most cost-effective approach for your research:

1. Detection Method (Biggest Cost Driver):

SYBR Green-based assays are more economical because they use a universal fluorescent dye and only require primer design ($50 per gene setup). TaqMan probe-based assays cost more due to custom probe synthesis but offer superior specificity and multiplexing capability ($225-$325 per gene). For most applications, SYBR Green provides excellent results at lower qPCR service costs.

2. Sample Number and Replication:

Per-sample costs ($16.50 for triplicate, $15 for duplicate wells) scale linearly with your sample size. However, SYBR Green assays become increasingly cost-effective with larger sample numbers since the $50 gene setup fee covers up to 1,000 samples. For studies with 50+ samples, SYBR Green offers significant savings compared to TaqMan.

3. Assay Availability:

Pre-validated, inventoried TaqMan assays cost less ($225/gene) than custom made-to-order designs ($325/gene) because no additional optimization is required. For well-characterized genes, inventoried assays provide excellent quality at lower qPCR service pricing.

4. Number of Genes Analyzed:

Multi-gene panels offer economies of scale. Our custom SYBR Green panels ($1,200 setup for up to 90 genes, $200/additional sample) provide exceptional per-gene value compared to analyzing genes individually. This makes pathway analysis and comprehensive gene profiling affordable.

5. Sample Preparation Requirements:

If you’re submitting cells, tissue, or specialized samples (FFPE, LCM, FNAB), RNA isolation ($40/sample) and quality assessment ($30/sample) add to total costs but ensure optimal results. Submitting pre-isolated, high-quality RNA reduces these expenses.

6. Turnaround Time:

Our standard 5-day turnaround is included in base pricing. Expedited service is available for a modest additional fee when research timelines demand faster results.

Cost-Saving Strategies:

To optimize your qPCR service costs: choose SYBR Green when specificity requirements allow, submit high-quality RNA when possible, combine multiple genes in panel format for large studies, and consult with our scientists about the most cost-effective approach for your specific project. We provide free consultations to help you balance cost and quality for optimal results.

Selecting the right qPCR service provider is critical for obtaining reliable, publication-quality gene expression data. Here are the key factors to evaluate:

1. Scientific Expertise and Credentials:

Look for a qPCR service provider whose staff holds advanced degrees (PhD-level) in molecular biology or related fields. At ARQ Genetics, ALL our research staff are PhD scientists with years of gene expression experience. This expertise ensures proper experimental design, accurate troubleshooting, and meaningful interpretation of results—not just mechanical sample processing.

2. Specialization vs. Generalist Approach:

Choose a provider specializing in qPCR rather than a generalist core facility offering dozens of different techniques. Specialized qPCR service providers maintain deeper expertise, optimize protocols specifically for gene expression analysis, and stay current with evolving best practices. We focus exclusively on real-time PCR services, allowing us to excel at what we do.

3. Quality Control and Validation:

A reputable qPCR service provider should run all experiments with proper controls (no-RT controls, no-template controls, positive controls), validate assay specificity, report amplification efficiency, and provide complete documentation. Ask about their QC procedures—transparency indicates quality commitment.

4. Instrumentation and Technology:

State-of-the-art qPCR systems ensure accurate, reproducible results. We utilize the 7900 HT and CFX-384 real-time PCR systems—industry-leading platforms for high-throughput gene expression analysis. Additionally, we’re an authorized TaqMan® Service Provider, indicating validated technical competency.

5. Flexibility and Customization:

Avoid qPCR service providers with rigid protocols or minimum order requirements. Your research should dictate the approach, not their convenience. Look for providers offering custom gene panel design, flexible sample sizes, both SYBR Green and TaqMan options, and willingness to adapt protocols to your specific needs.

6. Communication and Support:

A quality qPCR service provider offers free consultations, transparent communication throughout your project, accessibility for questions and troubleshooting, and publication-quality documentation. We maintain an open partnership approach—it’s not a “black box” service. You should feel supported from experimental design through manuscript preparation.

7. Turnaround Time:

Research timelines matter. We deliver most results within 5 business days, with expedited options available when deadlines are tight. Slow turnaround from a qPCR service provider can delay publications, grant submissions, and research progress.

8. Pricing Transparency:

Beware of hidden fees or unclear pricing structures. A trustworthy qPCR service provider provides upfront, itemized pricing so you can budget accurately. We offer transparent costs with no surprises, helping you make informed decisions about your research investment.

Red Flags to Avoid:

Be cautious of providers who: don’t specify staff credentials, refuse to discuss QC procedures, offer suspiciously low prices (often indicates cut corners), have inflexible protocols or high minimum orders, or provide poor communication. Quality qPCR analysis requires expertise and attention—extremely cheap services often deliver disappointing results.

Choosing the right qPCR service provider is an investment in your research success. We invite you to contact us for a free consultation to discuss your project and experience the ARQ Genetics difference.

As a specialized qPCR service provider, ARQ Genetics offers several distinct advantages that set us apart from both commercial contract research organizations and institutional core facilities:

Uncompromising Expertise:

ALL our research staff hold PhD degrees in molecular biology or related fields—not just technician-level training. Unlike many qPCR service providers where your samples may be processed by undertrained staff, you’re working with experienced scientists who understand experimental design, can troubleshoot complex issues, and contribute meaningfully to your research strategy. We’re scientific partners, not just a processing facility.

True Customization:

Many qPCR service providers restrict you to pre-defined gene panels or require minimum order quantities. We design multi-gene panels to YOUR specifications—choose exactly the genes you want to study, with no restrictions or minimum orders. Whether you need one gene or ninety, ten samples or a thousand, we tailor our services to your actual research needs, not our operational convenience.

Competitive Advantages vs. Core Facilities:

While institutional core facilities may offer subsidized pricing, they often have limitations: long wait times during peak periods, shared attention across multiple techniques, standardized protocols that may not be optimal for your samples, and limited consultation time. As a dedicated qPCR service provider, we offer faster turnaround (5 business days standard), specialized expertise in gene expression analysis, flexible protocols optimized for your needs, and responsive communication throughout your project.

Competitive Advantages vs. Large CROs:

Large contract research organizations may have extensive capabilities but often lack personal attention and flexibility. Unlike these larger qPCR service providers, we maintain direct scientist-to-researcher communication, accommodate mid-project adjustments as your research evolves, provide free consultations without sales pressure, and offer transparent pricing without hidden fees. You’re not just another sample number—we know your project and your goals.

Transparency and Partnership:

Unlike some qPCR service providers who operate as “black boxes,” we maintain transparent communication throughout your project. We keep you informed at every step, welcome your input and questions, provide complete documentation including raw data, and offer ongoing support even after results delivery. This partnership approach ensures you understand your data and can confidently present it in publications.

Cost-Effectiveness:

Our pricing is competitive with other commercial qPCR service providers while offering superior expertise and service. For multi-gene studies, our custom SYBR Green panels provide exceptional value compared to commercial platforms. We focus on delivering the best results for your investment, not maximizing our profit per sample.

Geographic Reach:

While we’re based in Bastrop, Texas, we serve researchers nationwide and internationally. Our streamlined sample shipping procedures and efficient communication ensure that distance doesn’t compromise service quality. Many of our long-term clients are from institutions across the country who value our expertise over proximity to a local provider.

We invite you to experience the ARQ Genetics difference. Contact us for a free consultation to discuss how our qPCR services can support your research goals, or request a detailed quote to compare our value proposition to other providers you’re considering.